BIOVISION Live-Dead Cell Staining Kit
BIOVISION Live-Dead Cell Staining Kit
BioVision的活-死細(xì)胞染色試劑盒提供了即用型試劑來(lái)方便地區(qū)分死細(xì)胞和活細(xì)胞。本染色試劑盒利用Live-Dye染料來(lái)對(duì)活細(xì)胞染色,這是一種能穿透細(xì)胞的綠色熒光染料(Ex/Em = 488/518 nm)。死細(xì)胞可以簡(jiǎn)單的用碘化吡啶(PI)染色,一種不能透過(guò)細(xì)胞膜的紅色熒光染料(Ex/Em = 488/615)。染上色的活細(xì)胞和死細(xì)胞通過(guò)使用了濾光片(檢測(cè)FITC和羅丹明)的熒光顯微鏡可以直接從視覺(jué)上區(qū)分開(kāi)來(lái)。本試劑盒提供的試劑可以在24孔板上染色100次。
BIOVISION Live-Dead Cell Staining Kit
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Kit Summary:
? Detection method- Fluorescent microscopy (Ex/Em 488/518 nm) to detect staines live cells; (Ex/Em 488/615) to detect stained dead cells
? Sample type- Cell culture (adherent and suspension cells)
? Species reactivity- Mammalian
? Applications- Stained live and dead cells can easily be visualized by fluorescence microscopy.
Features & Benefits:
? Simple procedure; takes less than 20 minutes
? Fast and convenient
Kit components:
? Solution A (1 mM Live-Dye)
? Solution B (2.5 mg/ml PI)
? Staining Buffer
Description:
Distinguishing between live and dead cells is very important for investigation of growth control and cell death. The Live-Dead Cell Staining Kit provides the ready-to-use reagents for convenient discrimination between live and dead cells. The kit utilizes Live-Dye?, a cell-permeable green fluorescent dye (Ex/Em = 488/518 nm), to stain live cells. Dead cells can be easily stained by propidium iodide (PI), a cell non-permeable red fluorescent dye (Ex/Em = 488/615). Stained live and dead cells can be visualized by fluorescence microscopy using a band-pass filter (detects FITC and rhodamine). The kit provides sufficient reagents for 100 stainings using 24-well plate.
Storage Conditions:
-20°C
Shipping Conditions:
gel pack
USAGE: For Research Use Only! Not For Use in Humans.
Biovision活死細(xì)胞雙染試劑盒Live-Dead Cell Staining Kit